Scientists Find One Way Antibiotic Resistance Develops

11/24/2012 7:00 AM

PULLMAN, Wash. — Washington State University researchers have found an unlikely recipe for antibiotic resistant bacteria: Mix cow dung and soil, then add urine infused with metabolized antibiotic.

The urine will kill off normal E. coli in the dung-soil mixture. But antibiotic-resistant E. coli will survive in the soil to recolonize in a cow’s gut through pasture, forage or bedding.

“I was surprised at how well this works, but it was not a surprise that it could be happening,” said Doug Call, a molecular epidemiologist in WSU’s Paul G. Allen School for Global Animal Health.

Call led the research with a doctoral student in immunology and infectious disease, Murugan Subbiah, now a post-doctoral researcher at Texas A & M. Their study appeared in a recent issue of the online journal PLOS ONE.

While antibiotics have dramatically reduced infections in the past 70 years, their widespread use has led to the natural selection of drug-resistant microbes. People infected with the organisms have a harder time getting well, with longer hospital stays and a greater likelihood of death.

Many people believe animals are a major source of resistant bugs because they receive the bulk of antibiotics sold in the U.S.

The scientists focused on the antibiotic ceftiofur, a cephalosporin believed to be helping drive the proliferation of resistance in such bacteria as salmonella and E. coli. Ceftiofur has little impact on gut bacteria, Call said.

“Given that about 70 percent of the drug is excreted in the urine, this was about the only pathway through which it could exert such a large effect on bacterial populations that can reside in both the gut and the environment,” he said.

Until now, conventional thinking held that antibiotic resistance is developed inside the animal, Call said.

“If our work turns out to be broadly applicable, it means that selection for resistance to important drugs like ceftiofur occurs mostly outside of the animals,” he said. “This in turn means that it may be possible to develop engineered solutions to interrupt this process.

“In doing so, we would limit the likelihood that antibiotic resistant bacteria will get back to the animals and thereby have a new approach to preserve the utility of these important drugs,” he said.

One possible solution would be to find a way to isolate and dispose of residual antibiotic after it is excreted from an animal but before it interacts with soil bacteria.

The experiments were performed in labs using materials from dairy calves. Researchers must now see if the same phenomenon takes place in actual food-animal production systems.

Source: American Association for the Advancement of Science.


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